Abstract
1. When UV-irradiated in vacuum (10−6 Torr), stationary phase cells ofE. coli B/r showed an increased UV sensitivity regarding the inactivation of colony forming ability. The enhancement factor of theD10 values was 12.8, that of the slopes of the survival curves was 6.1. This increased UV sensitivity in vacuum was not caused by selection of UV-sensitive mutants by vacuum treatment. Photoenzymatic repair of the lethal UV lesions obtained in vacuum was not detectable, though the photoreactivating enzyme was shown to be intact. 2. In contrast there was no enhanced sensitivity concerning the delay of the first cell division after UV irradiation in vacuum. The division delay was additive regarding the single effects of UV and vacuum.
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