Inactivating Gene Expression with Antisense Modified Oligonucleotides.

Sidney Altman,Carlos Angele-Martinez

doi:10.32607/actanaturae.11522

Abstract

Modified nucleotides, including phosphoramidates and mesyl nucleotides, are very effective in inactivating gene expression in bacteria. Gyr A is the target gene in several organisms, including Plasmodium falciparum. Antisense reactions with bacteria infecting citrus plants are promising but incomplete. Human tissue culture cells assayed with a different target are also susceptible to the presence of mesyl oligonucleotides.

Highlights

Over several years, the research focused on RNase P has been relying on standard oligonucleotides A, C, U, G in RNA (Fig. 1)
Once the focus switched to the study of the suppression of the activity of various genes, the advent of phosphoramidates (PMs; [6, 7]; see Fig. 2) and 2’OMe nucleotides, which have the advantage of being characterized by a higher membrane permeability and nuclease resistance compared to those of standard oligonucleotides, has led to a spate of attempts using modified oligonucleotides (MOs) as antisense oligonucleotides to turn gene expression off
To show cleavage of the gyr A sequence, the Wolbachia sequence (Table 1), the MO, and the gyr RNA were exposed to M1 RNA, as well as to the purified E. coli RNase P

Summary

Introduction

INTRODUCTION Over several years, the research focused on RNase P has been relying on standard oligonucleotides A, C, U, G in RNA (Fig. 1). Various permutations of MOs (i.e., using different modified oligos at various positions in the antisense molecules) proved unsuccessful in gene inactivation studies.

Results

Conclusion