Inactivated Sendai virus strain Tianjin induces apoptosis in human breast cancer MDA-MB-231 cells.

Jun Chen,Li-Ying Shi,Bin Wang,Han Han,Min Chen,Xiao-Zhu Xu

doi:10.7314/apjcp.2014.15.12.5023

Jun Chen, Li-Ying Shi + Show 4 more

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https://doi.org/10.7314/apjcp.2014.15.12.5023

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Abstract

Sendai virus strain Tianjin is a novel genotype. Here, we investigate the antitumor and proapoptotic effects of ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) on human breast cancer MDA-MB-231 cells in vitro, as well as the involvement of the apoptotic pathway in the mechanism of UV-Tianjin-induced antitumor effects. MTT assays showed that treatment with UV-Tianjin dose-dependently inhibited the proliferation of MDA- MB-231 cells but not normal MCF 10A breast epithelium cells. Hoechst staining and flow cytometric analysis revealed that UV-Tianjin induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Moreover, UV-Tianjin treatment resulted in reduction in the mitochondria membrane potential (MMP) and release of cytochrome complex (cyt c) via regulation of Bax and Bcl-2, as well as activation of caspase-9, caspase-3, Fas, FasL and caspase-8 in MDA-MB-231 cells. In summary, our study suggests that UV-Tianjin exhibits anticancer activity in human breast cancer MDA-MB-231 cells through inducing apoptosis, which may involve both the endogenous mitochondrial and exogenous death receptor pathways.

Highlights

Breast cancer is a life-threatening disease among women worldwide with the rate of reported incidence and mortality increasing annually (Jemal et al, 2011; Ibrahim et al, 2013)
The results demonstrated that a visible increase in Fas, FasL, and cleaved caspase-8 was observed after UV-Tianjin treatment at multiplicity of infection (MOI) 80, as compared with control cells and cells treated with 20 MOI of UV-Tianjin (Figure 4B)
We demonstrated that UVTianjin significantly inhibited the proliferation of human breast cancer MDA-MB-231 cells in a dose-dependent manner

Summary

Introduction

Breast cancer is a life-threatening disease among women worldwide with the rate of reported incidence and mortality increasing annually (Jemal et al, 2011; Ibrahim et al, 2013). Ultraviolet-inactivated, replication-defective Sendai virus [hemagglutinating virus of Japan envelope (HVJ-E)] is considered to be a safe and efficient nonviral vector for drug delivery because it can incorporate DNA, RNA, proteins, or drugs and deliver them into cells (Kaneda, 2003; Ito et al, 2005; Kaneda et al, 2005; Zhang et al, 2008). Ultraviolet-inactivated Tianjin strain (UV-Tianjin) suppressed the growth of colon carcinomas in mice by inducing an antitumor immune response and tumor-cell apoptosis (Shi et al, 2013). Asian Pacific Journal of Cancer Prevention, Vol 15, 2014 5023 investigate the effect of UV-Tianjin on the proliferation and apoptosis of the human breast cancer cell line MDAMB-231 in vitro, and the possible mechanism

Materials and Methods

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Discussion