Inaccuracy in Preparation of Intravitreal Solutions-Reply

Abstract

In Reply. —We agree with Dr Noske that the large amount of dead space found in many syringes can be a source of inaccurate dilutions. As mentioned in the Comment section of our paper, we took care to use a hubless 27-gauge insulin syringe to make our dilutions. We think this seemingly minor technical point is important because a hubless syringe has minimal dead space. Our article originally contained a photograph (Figure) of this syringe that was later omitted to conserve space. Before we submitted our article, we rechecked the accuracy and reproducibility of dilutions using this type of syringe. We used solutions of fluorescein in a manner similar to the use of glucose solutions by Dr Noske. We did not submit the resulting data in our article because of space considerations. In this endeavor, we first tried our original method, that of initially drawing in 0.1 mL of the