Abstract
Chemotherapy treatments induce a number of side effects, such as leukopenia neutropenia, peripheral erythropenia, and thrombocytopenia, affecting the quality of life for cancer patients. 5-Fluorouracil (5-FU) is wieldy used as myeloablative model in mice. The bovine dialyzable leukocyte extract (bDLE) or IMMUNEPOTENT CRP® (ICRP) is an immunomodulatory compound that has antioxidants and anti-inflammatory effects. In order to investigate the chemoprotection effect of ICRP on bone marrow cells in 5-FU treated mice, total bone marrow (BM) cell count, bone marrow colony forming units-granulocyte/macrophage (CFU-GM), cell cycle, immunophenotypification, ROS/superoxide and Nrf2 by flow cytometry, and histological and hematological analyses were performed. Our results demonstrated that ICRP increased BM cell count and CFU-GM number, arrested BM cells in G0/G1 phase, increased the percentage of leukocyte, granulocytic, and erythroid populations, reduced ROS/superoxide formation and Nrf2 activation, and also improved hematological levels and weight gain in 5-FU treated mice. These results suggest that ICRP has a chemoprotective effect against 5-FU in BM cells that can be used in cancer patients.
Highlights
Most of chemotherapeutic agents can cause myelosuppression in a dose-dependent manner [1]
Bovine dialyzable leukocyte extract or IMMUNEPOTENT CRP (ICRP) is a dialysate of a heterogeneous mixture of low-molecular-weight substances released from disintegrated leukocytes of the blood or lymphoid tissue obtained from homogenized bovine spleen
We examined the effects of ICRP on 5-FU treated mice on red blood cell (RBC), hemoglobin (HB), hematocrit (HCT), white blood cell (WBC), and platelets (PLT) levels 1 and 7 days after initiating treatments
Summary
Most of chemotherapeutic agents can cause myelosuppression in a dose-dependent manner [1]. Journal of Immunology Research with neutropenia, mucositis, leukopenia, and hematological toxicity [9, 10]. Because of these side effects, chemoprotective compounds have been used to reduce these problems [11,12,13,14,15,16,17,18,19]. ICRP was capable of stimulating the immune system in patients with non-small cell lung cancer and increasing their quality of life [20]. In vitro studies demonstrated that ICRP was an effective therapeutic agent in process involving oxidative cellular damage and clinical inflammatory diseases, through IκB/NF-κB pathway [21]. We examined the protector effect of ICRP on myelosuppression caused by 5-FU in a mouse model
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