Abstract
A bioluminescent phenotype of Escherichia coli was employed to follow bacterial uptake onto model polystyrene surfaces. Attachment from suspension was rapid and the isotherm exhibited Langmuirian characteristics. The uptake pattern was reflected in both real‐time bioluminescence measurement of attached bacteria in a luminometer and in conventional viable count determination. Treatment of attached bacteria with 3 biocidal agents produced a rank order of efficacy of Proxel GXL (0.01%)<chlorhexidine diacetate (0.0015%) < phenol (0.5%), an order confirmed by both bioluminescence and viable counts.
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