Abstract
Photoaging is known to be correlated with different kinds of oxidative stress. Accumulation of lipid peroxidation, products of glycation, and protein oxidation are often used as biomarkers for oxidative stress. Protein carbonyl may be formed by oxidative cleavage of proteins or direct oxidation of lysine, arginine, praline, and threonine residues. Its level could be detected by immunostainingand used as an indicator for the extent of reactive oxygen-mediated protein oxidation. It have been reported this biomarker have been used for detecting a higher extent of oxidation of human stratum corneum keratin 10 in the outer layer of epidermis (stratum corneum) than in the inner layer of epidermis (keratinocyte). A simple immunoanalysis protocol have been set up based on detecting the extent of protein oxidation. This could facilitate the establishment of an in vivo evaluation platform for anti-oxidative cosmetics. The optimal experiment conditions including extraction of scalp proteins, the reaction of DNPH (2, 4-dinitrophenylhydrazine) with carbonyl, and antibody reactions have been identified. This provides a viable way for detecting the oxidation of scalp proteins. This method could be used in evaluating anti-oxidative cosmetic formulations.
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