Abstract
Bone loss associated with musculoskeletal trauma or metabolic diseases often require bone grafting. The supply of allograft and auto-graft bones is limited. Hence, development of synthetic bone grafting materials is an active area of research. Chitosan, extracted from chitin present in crawfish shells, was tested as a de-livery vehicle for osteoblasts in a 2-3 mm size defect model in rats. Twenty-seven male Lewis rats, divided into three groups with sacrifice intervals of 3, 6 & 9 months were used. In the experimental samples, a critical size defect was filled with chitosan bone graft paste and fixed with a plate, while in the operated control group, a critical size defect was repaired only by a plate (no paste was applied). An unoperated control group was also included. Bone growth was evaluated histologically by examining undecal-cified and decalcified stained sections. The fe-murs were also examined non-destructively by micro-computed tomography (礐T). Defects filled with chitosan bone graft paste demon-strated superior healing across all time periods compared to unfilled defects as examined by histology and micro-computed tomography. Crawfish chitosan has successfully been used as a cell delivery system for osteoblasts for use as a synthetic bone graft material.
Highlights
Bone loss associated with musculoskeletal trauma or metabolic diseases often require bone grafting
More bone was present in the experimental group compared to the operated control group
During the three month interval, 40.23% bone growth was calculated in the experimental group while only 29.43% was calculated in the operated control group
Summary
Bone loss associated with musculoskeletal trauma or metabolic diseases often require bone grafting. Development of synthetic bone graft materials is an active area of research. Chitin is a polysaccharide that exists in fungi, exoskeleton of insects and the outer shell of crustaceans [1,2,3]. Chitosan has been investigated in a number of biomedical applications due to its purity coupled with a positive charge [13,14,15,16]. This positive charge interacts with cells or can act as a binding site for other functional groups thereby expanding the role of the chitosan molecules
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
