Abstract
Glucosinolates are amino acid-derived, natural plant products found in the order Capparales. Radiolabelled glucosinolates for use in biological studies concerning metabolism and transport are not commercially available. We have developed a method for the synthesis and isolation of radiolabelled p-hydroxybenzylglucosinolate (p-OHBG) produced in vivo by Sinapis alba, which has the tyrosine-derived p-OHBG as its major glucosinolate. The method is based on administration of radiolabelled tyrosine to jasmonate-treated primary leaves, which have high de novo biosynthesis of p-OHBG. After incubation, the plant material was extracted with methanol and the glucosinolates purified by a procedure including two ion exchange chromatographic steps. The method provides an attractive alternative to chemical synthesis of radiolabelled glucosinolates and is applicable to other plants producing a single or one major glucosinolate. Copyright © 2000 John Wiley & Sons, Ltd.
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