Abstract
Vibrio vulnificus isolated from diseased Mullet, Mugil cephalus L., were immunized with formalin inactivated Vibrio vulnificus to the juveniles of priming and booster groups of fish. Assessing the humoral immune response using the serum agglutination assays. This experiment was performed to compare the antibody titre in priming dose and booster dose. The result revealed that the higher priming response at 21 dpp and booster response at 35 dpb. This intramuscular injection was more suitable for inducing the primary response than intraperitoneal injection.
Highlights
Aquaculture is growing rapidly world wide with fish being the primary source of animal protein in many countries
Collado et al (2000) studied the effectiveness of different vaccines for Vibriosis caused by Vibrio vulnificus bio type 2 in European eels
The collected samples like blood, kidney tissues were inoculated in suitable culture medium such as Zobell’s Marine agar, alkaline peptone water, & Thiosulfate Citrate Bile salt Sucrose (TCBS) agar
Summary
Aquaculture is growing rapidly world wide with fish being the primary source of animal protein in many countries. The lack of understanding of intricate balance between the host, pathogen and the environment was most obvious. These conditions, causes the organisms to be more prone to infections. The development of drug resistance of fish pathogens has frequently been reported (Aoki, 1992).Virbriosis has been a major cause of concern especially in brackish water & marine aquaculture systems. Collado et al (2000) studied the effectiveness of different vaccines for Vibriosis caused by Vibrio vulnificus bio type 2 in European eels. The various studies revealed that several substances have been tested for the prevention of bacterial diseases including whole cell bacteria (Fukuds & Kusuda, 1981), attenuated live vaccines (Kusuda and Hamaguchi, 1988), VIJAYALAKSHMI et al, Curr. In the present study vaccination of Mugil cephalus against Vibrio isolate and consequent analysis of several antibody titres were performed
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