Abstract
The present study was designed to examine whether thyroid stimulating antibodies (TSAb) against the mutated or non-mutated peptide of human TSH receptor stimulates release of thyroid hormones in mice in vivo. We generated antibodies against mutated TSH receptor peptide TSH-R-HIS 17 and non-mutated TSH receptor peptide TSH-R-ASP 17 immunizing the peptides to rabbits. We isolated immunoglobulin G (lgG) from these antibodies and injected iv into male ddy mice. Control mice were injected iv with bTSH or TSAb-negative normal rabbit lgG. Serum T3 and T4 concentrations were elevated after the administration of bTSH in a dose-dependent manner. Serum T3 and T4 concentrations were significantly elevated after the injection of lgG from the rabbit antibodies with TSAb activity produced against TSH-R-HIS 17. However serum T3 and T4 remained within normal levels after the injection of lgG from the rabbit antibodies without TSAb activity toward TSH-R-ASP 17. The present data offer in vivo support of the lgG with TSAb activity interaction with the TSH receptor of the thyroid and raise the possibility that TSAb against mutated human TSH receptor peptide might be contributed to induce thyroid hyperfunction.
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