In Vivo Solid‐Phase Microextraction: Capturing the Elusive Portion of Metabolome

Abstract

Metabolites with fast turnover rates and/or unstable metabolites can be captured directly from circulating blood using a new solid-phase microextraction (SPME) device based on a hypodermic needle. Such metabolites are not observed using solvent precipitation and ultrafiltration methods, indicating traditional approaches based on blood withdrawal are not adequately capturing complete metabolome at the time of sampling. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.