Abstract
Purpose We describe a method for measuring the pharmacokinetics of positron emission tomography (PET) tracers during rat studies using microdialysis (MD) in combination with planar chromatography and digital autoradiography with a phosphoimager plate (DAR). Procedures An MD probe was inserted into the jugular vein of the rat and perfused with physiological buffer solution (n≥3). An 18F-labeled radiopharmaceutical was injected intravenously, and dialysate fractions were collected, measured for radioactivity, and applied on a chromatography plate. This plate was exposed with an imaging plate for radioactivity imaging. Results Time activity curves for unbound, unchanged tracer and metabolites were determined. The amount of unchanged tracer in the last MD fraction was compared with the amount in the plasma sample collected after sacrifice of the animal. Conclusion MD continuously samples and monitors the amount of unbound tracer in the blood from one single experimental animal. MD combined with planar chromatography and DAR is a highly sensitive and linear method for simultaneous analysis of PET tracers and their metabolites in blood.
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