Abstract
When E. coli C cells, infected with UV irradiated ΦX 174, were allowed to grow in liquid tris-glucose medium at 37° C with aeration, the UV damage of the single stranded (ss) DNA could be repaired to some extent. Such repair was not possible if the irradiated phage were plated immediately on E. coli C in the usual double layer agar method, or if the infected complexes were initially exposed to 0.02 M KCN for 15 min before they were allowed to grow in tris-glucose medium as before. Our results indicate that in order to be repaired, ss DNA containing UV damage must be able to convert itself to a closed circular double stranded replicative form (RF) within the host cells escaping prior scission. The whole process of repair was found to be dependent on protein synthesis in the infected complexes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
