Abstract
The functional role of AMPA receptor (AMPAR)-mediatedsynaptic signaling between neurons and oligodendrocyte precursor cells (OPCs) remains enigmatic. We modified the properties of AMPARs at axon-OPC synapses in the mouse corpus callosum invivo during the peak of myelination by targeting theGluA2 subunit. Expression of the unedited (Ca2+ permeable) or the pore-dead GluA2 subunit of AMPARs triggered proliferation of OPCs and reduced their differentiation into oligodendrocytes. Expression of the cytoplasmic C-terminal (GluA2(813-862)) of the GluA2 subunit (C-tail), a modificationdesignedto affect the interaction between GluA2 and AMPAR-binding proteins and to perturbtrafficking of GluA2-containing AMPARs, decreased thedifferentiation of OPCs without affecting their proliferation. These findings suggest that ionotropic and non-ionotropic properties of AMPARs in OPCs, aswell as specific aspects of AMPAR-mediated signaling at axon-OPC synapses in the mouse corpus callosum, are important for balancing the response of OPCs to proliferation and differentiation cues.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have