Abstract
The expression of cochlin in the trabecular meshwork (TM) precedes the clinical glaucoma symptoms in DBA/2J mice. The ability to quantify cochlin in the local tissue (TM) offers potential diagnostic and prognostic values. We present two (spectroscopic and magnetomotive) optical coherence tomography (OCT) approaches for in vivo cochlin quantification in a periodic manner. The cochlin-antibody OCT signal remains stable for up to 24 hours as seen at 3.5 hours after injection allowing for repeated quantification in the living mouse eyes.
Highlights
We found an incubation of ~3.5 hours achieves uniform antibody spreading with every 90° injection (4 injections total) for DBA/2J mice with a range of cochlin in their trabecular meshwork (TM) (Fig. 1b)
In an effort to quantify total cochlin localized in the TM, we preferred to match the stacks of images before and after antibody injections for both SOCT and MMOCT modalities
Our results are consistent with degradation of the cochlin-antibody complex predominantly in the extracellular matrix (ECM) (Fig. 3c) Our results suggests the periodicity of the cochlin measurement will need at least a 72 hours gap period between first determination and injection for subsequent quantification
Summary
This was evaluated in vitro using polymeric spheres with or without injection of the antigen-antibody complex (Supplementary Fig. 1e). These spheres were used for MMOCT imaging with different amounts of cochlin-antibody complex (Supplementary Fig. 1g).
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