Abstract
This paper offers a study of the production of macrophage migration inhibition factor (MIF), and also of the alternative macrophage migration stimulation factor (MSF), in vivo. Mice were injected with mouse spleen cells, irradiated with a dose of 1500 rads. The animals were divided into three groups, two of which were injected for a second time with irradiated mouse spleen cells. Samples of all fractions obtained by electrophoresis of sera of unimmunized mice had no significant effect of macrophage migration, while unfractionated sera of immunized mice obtained after a second injection of alloantigen as a rule stimulated macrophage migration. The results are evidence that T cells may function in vivo during the period before development of the antigen-specific proliferative response of T cells. The technique used to approach the problem, described in this study, can be used for preparative isolation of purified MIF and MSF without contamination by embryonic calf serum proteins which are usually present in culture in vitro.
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