Abstract
The detection of neuroelectrophysiology while performing optogenetic modulation can provide more reliable and useful information for neural research. In this study, an optical fiber and a microelectrode array were integrated through hot-melt adhesive bonding, which combined optogenetics and electrophysiological detection technology to achieve neuromodulation and neuronal activity recording. We carried out the experiments on the activation and electrophysiological detection of infected neurons at the depth range of 900–1250 μm in the brain which covers hippocampal CA1 and a part of the upper cortical area, analyzed a possible local inhibition circuit by combining opotogenetic modulation and electrophysiological characteristics and explored the effects of different optical patterns and light powers on the neuromodulation. It was found that optogenetics, combined with neural recording technology, could provide more information and ideas for neural circuit recognition. In this study, the optical stimulation with low frequency and large duty cycle induces more intense neuronal activity and larger light power induced more action potentials of neurons within a certain power range (1.032 mW–1.584 mW). The present study provided an efficient method for the detection and modulation of neurons in vivo and an effective tool to study neural circuit in the brain.
Highlights
Optogenetic technology has been widely used in the research of behavior, diseases and other aspects of neural mechanism since it was put forward in 2005 [1], and on the central nervous system; it had effects on the peripheral nervous system [2,3]
The neuronal in a large range from to μm in the brain) under optogenetic control were detected and the infected neurons under optogenetic control were detected and the infected were successfully activatedactivated by opticalbystimulation, which proved theproved excellent of the neurons were successfully optical stimulation, which theperformance excellent performance integrated device
A possible circuit is analyzed by optogenetic modulation and and of the integrated device
Summary
Optogenetic technology has been widely used in the research of behavior, diseases and other aspects of neural mechanism since it was put forward in 2005 [1], and on the central nervous system; it had effects on the peripheral nervous system [2,3]. Optogenetic technology can modulate the activities of target neuron cells with high spatial-temporal resolution by combining photonics and genetic techniques and has been one of the most important neuroscience research tools [4,5,6]. The core of optogenetics is light-sensitive protein, called opsins, which are mainly found in micro-organisms. The opsin gene is introduced into the subject’s brain through viral vectors, and is eventually expressed in targeted cells after transcription [7,8]. The light-sensitive protein expressed in the cell membrane acts as an ion channel or pump controlled by light in a specific wavelength range.
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