Abstract
In the previous research, we obtained three catagories of M1 mutant cowpea namely 1) purple pod mutant cowpea, 2) green pod mutant cowpea which has three pods in a stalk, and 3) green pod mutant cowpea which has two pods in a stalk. All those three catagories of M1 mutant cowpeas were treated again with colchicine in a multistep mutagenesis proses in vivo. Then, we found changes in number of leaflet in a petiole to 4 leaflets (quadrifoliate) and to five leaflets in a petiole (pentafoliate). These changes are indications that beside as a chromosome multipying alkaloid, colchicine causes other genetic changes as well. Through this mutagenesis research, we created gigas mutant cowpea that having longer leaves, longer pods and heavier seeds. The accurrence of two kinds of insect pests attack were found namely cowpea aphid Aphis craccivora and pod sucking bug Riptortus linearis.
Highlights
Cowpea is a kind of beans that suitable for tropical climate and it grow well even in semi-arid regions (Timko and Singh, 2008)
Mutant cowpeas that were grown from purple pod mutant cowpea having leaf size (6,5±0,59cm) smaller than green pod mutant cowpea (10,6±1,84cm) i.e. 39% smaller
All purple pod mutant cowpeas were attacked with cowpea mosaic virus (CPMV) and failed to produce pod, whereas green pod mutant cowpea, with two or three pods per stalk weren’t attacked with CPMV and succesfully produced pods
Summary
Cowpea is a kind of beans that suitable for tropical climate and it grow well even in semi-arid regions (Timko and Singh, 2008). All those three catagories of M1 mutant cowpeas were treated again with colchicine in a multistep mutagenesis proses in vivo. We created gigas mutant cowpea that having longer leaves, longer pods and heavier seeds.
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