Abstract
Although human intervertebral disc degeneration can lead to several spinal diseases, its pathogenesis remains unclear. This study aimed to create a new histological classification applicable to an in vivo mouse intervertebral disc degeneration model induced by needle puncture. One hundred six mice were operated and the L4/5 intervertebral disc was punctured with a 35- or 33-gauge needle. Micro-computed tomography scanning was performed, and the punctured region was confirmed. Evaluation was performed by using magnetic resonance imaging and histology by employing our classification scoring system. Our histological classification scores correlated well with the findings of magnetic resonance imaging and could detect degenerative progression, irrespective of the punctured region. However, the magnetic resonance imaging analysis revealed that there was no significant degenerative intervertebral disc change between the ventrally punctured and non-punctured control groups. To induce significant degeneration in the lumbar intervertebral discs, the central or dorsal region should be punctured instead of the ventral region.
Highlights
Human intervertebral disc (IVD) degeneration is a common cause of low back pain and it affects the daily activities [1,2,3]
The research focusing on IVD degeneration, from the gross anatomical to histological studies, has been conducted using various animal models: scalpel incision to annulus fibrosus (AF) in canines [4] and rats [5]; surface incision to AF in sheep [6]; full puncture to IVD using needle in mice[7] and rats [8]; and hemi-AF puncture using needle in mice [9], rats [10], and PLOS ONE | DOI:10.1371/journal.pone
The number of reports describing IVD degeneration in terms of the genetic approaches is increasing [1,15,16,17,18,19], there has been no appropriate histological classification applicable to an in vivo mouse intervertebral disc degeneration model, which is applicable to genetically modified mice
Summary
Human intervertebral disc (IVD) degeneration is a common cause of low back pain and it affects the daily activities [1,2,3]. It is the cause of spinal diseases such as spinal canal stenosis, disc herniation, and spinal deformity. To establish an IVD degeneration model induced by needle puncture, a precise size and shape of the device and a detailed procedure for each of the species are necessary. The aim of this study was to create a new histological classification applicable to an in vivo mouse intervertebral disc degeneration model induced by needle puncture
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
