Abstract
Because of its similar genetic makeup with humans, zebrafish are an available and well-established osteoporosis model in vivo for anti-osteoporosis drug development as well as the drug safety-evaluation process. However, few optical imaging methods could effectively visualize the bone of adult zebrafish due to their limited penetration depth. In this paper, in vivo high-resolution and long-term characterization of a prednisolone-induced osteoporotic zebrafish model was achieved with spectral-domain optical coherence tomography (SD-OCT). The capability of three-dimensional SD-OCT imaging was also demonstrated in this study. With SD-OCT images, we could non-destructively monitor the deforming process of adult zebrafish skull from several directions at any time. There is good correlation and agreement between SD-OCT and histology. Valuable phenomenon such as bone defects could be quantitatively evaluated using the SD-OCT images at different time points during a period of 21 days.
Highlights
Osteoporosis is physiologic or pathologic loss of mineralized tissues resulting in metabolic disease, making it a chronic degenerative disease endangering the health of human
Histological sectioning and staining has insurmountable deficiency, mainly including all the animals have to be sacrificed, which will bring significant challenges in the study of biological procedures in vivo. Optical imaging techniques such as confocal microscopy, two photon microscopy and light sheet microscopy have become increasingly popular in biological study based on zebrafish
A SD-Optical coherence tomography (OCT) system was employed for non-invasive characterizing the morphology of osteoporotic adult zebrafish model with high resolution
Summary
Osteoporosis is physiologic or pathologic loss of mineralized tissues resulting in metabolic disease, making it a chronic degenerative disease endangering the health of human. A SD-OCT system was employed for non-invasive characterizing the morphology of osteoporotic adult zebrafish model with high resolution. 3. Experimental results 3.1 System’s resolution and imaging depth Before in vivo experiment, we characterized the performance of the OCT system first.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
