Abstract
The interaction of circulating HGH with subcellular structures of female rat liver was studied in the intact animal. Various subcellular fractions including plasma membranes (Biochim. Biophys. Acta 154 : 540, 1968) and Golgi fractions (J. Cell. Biol. 59 : 45, 1973) were prepared from liver homogenates at different time intervals after a single injection of 125I-HGH into a peripheral vein. Labeling of plasma membranes occurred within the first minutes following injection, whereas labeling of Golgi fractions was delayed and reached a maximum by 15 minutes. Labeling of both subcellular structures was inhibited by excess (500 μg) native HGH but was unaffected by insulin. At all times studied virtually no radioactivity was found in the nuclear and mitochondrial-lysozomal fractions. The 125I-HGH eluted from the plasma membranes and from Golgi fractions appeared intact as tested by TCA precipitation and rebinding to rat liver membrane. The results suggest that, following binding to the surface of liver cells, HGH is translocated into Golgi elements under a biologically active form.
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