Abstract
Abstract T regulatory cells (Tregs) control peripheral tolerance, and their immunosuppressive function is essential to maintain a balanced immune system. Tregs constitutively express IL-2Rα (CD25) and the transcription factor FoxP3. IL-2 is a pleiotropic cytokine and is vital for the development of Tregs. IL-2 binds to the trimeric receptor formed by IL-2Rα, IL-2Rβ (CD122), and the common gamma chain IL-2Rg (CD132). In this study we induced in vivo Treg (CD25bright, FoxP3+, CD4+) expansion by injecting a complex made of recombinant mouse IL-2 and anti-mouse CD25 (clone JES6-1A12). The mice were injected for four consecutive days with different concentrations of IL-2_JES6-1A12 complex, as well as IL-2_JES6-1A12 complex plus an excess of IL-2, or IL-2 alone. There was a significant induction of Tregs in mice that received the complex as compared to animals that received only a comparable dose of IL-2 alone. The ratio Tregs/effector cells (defined as CD8+,CD44+, CD122+) was approximately two fold in mice that received the complex. Mice injected with the complex plus an excess of IL-2 showed similar proportions of Tregs and effector cells. Previous studies showed that the use of a different IL-2 neutralizing clone (clone S4B6, recognizes and block CD122) preferentially induced effector cells. This experimental model will facilitate the study of diseases or immune mechanisms where Tregs mediate an immune response or immune system dysregulation.
Published Version
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