Abstract
BackgroundCryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy.MethodsOvaries of donor FVB/N-Tg (PolII–Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the back muscles of recipient FVB/NJNarl wild-type mice that had undergone bilateral oophorectomy. We evaluated the fate of cryopreserved murine ovarian grafts by in vivo bioluminescent imaging (BLI), AMH mRNA expression and follicle counts.ResultsThere were significantly stronger BLI signals in the fresh ovaries than in the frozen–thawed ones. The number of primordial follicles was significantly lower in frozen–thawed ovaries at 10 days after transplantation (P < 0.001). The AMH mRNA expression was significantly lower in the frozen–thawed ovaries (P < 0.001), showing that unavoidable harm occurs after transplantation.ConclusionsOvarian cryopreservation by slow freezing compromises ovarian reserve by cryoinjury and ischemia, evident at an early stage after transplantation.
Highlights
Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy
Cryopreservation of ovarian tissue has been conducted since the 1990s and has been suggested as an alternative to restore fertility for girls and women who are at high risk for ovarian failure after chemotherapy or radiotherapy [1,2,3]
Our objective is to evaluate the fate of cryopreserved murine ovarian grafts by using in vivo bioluminescent imaging (BLI) in real time, based on longitudinal tracking of the individual subjects quantitatively in the short term, and by measuring the expression of Anti-Müllerian hormone (AMH) mRNA using real-time quantitative polymerase chain reaction amplification and follicle counts as the evidence whether the ovarian cryopreservation by slow freezing method was compromised the ovarian reserve at an early stage after transplantation
Summary
Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy. Cryopreservation of ovarian tissue has been conducted since the 1990s and has been suggested as an alternative to restore fertility for girls and women who are at high risk for ovarian failure after chemotherapy or radiotherapy [1,2,3]. This technique has several advantages over the simple cryopreservation of oocytes and embryos. Cryopreservation of ovarian tissue can preserve the endocrine functions of the ovary, which cannot be achieved by cryopreserving the oocyte or embryo This strategy is suitable for prepubertal girls [4,5]. A measure of AMH can be applied to the clinical evaluation of follicular reserves and function [11,12,13,14]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
