In vivo evaluation of 80% methanolic leaves crude extract and solvent fractions of buddleja polystachya fresen (buddlejaceae) for wound healing activity in normal and diabetic mice

Abstract

In diabetes mellitus, the wound healing process is impaired and delayed. Plants are actively investigated for safe, effective, and well-tolerated treatment options for wound. The plant Buddleja polystachya has a traditional claim for the treatment of wound. Fresh leaves are crushed and given for topical application. The objective of this study was to evaluate the wound healing activity of hydro-alcoholic extract and solvent fractions of leaves of B. polystachya fresen (Buddlejaceae) in normal and diabetic mice. MethodB. polystachya leaves were extracted using 80% methanol and further fractionated using chloroform, ethyl acetate, and water. The wound healing activity was tested using excision, incision, and diabetic wound models. Area of wound contraction, time of epithelialization, and wound breaking strength were determined. ResultTreatment with 5% w/w (P < 0 0.05) and 10% w/w (P < 0.001) crude extract of B. polystachya significantly lowered wound area as compared to simple ointment. Treatment of excision wound with 5% w/w and 10% w/w of chloroform, ethyl acetate, and aqueous fractions of B. polystachya significantly lowered wound area and epithelization period (P < 0.05). The 10% w/w extract of B. polystachya showed a significant area of diabetic wound contraction compared to 5% w/w extract and simple ointment. The 10% w/w and 5% w/w extracts of B. polystachya significantly increased wound breaking strength compared to untreated and simple ointment groups (P < 0.05). ConclusionThe crude and the solvent fractions of B. Polystachya leaves possess wound healing activities as evidenced by an increase in tensile strength and wound contraction rate and decrease in the epithelialization period. This finding supports the traditional claims of B. polystachya for the healing of wounds.