Abstract
Specimens of Crassostrea virginica were exposed to 1 ppm Cu 2+, Cd 2+, Cu 2+ + EDTA, Cd 2+ + EDTA, or sea water for 24 hr, 72 hr, 1 week, and 2 weeks. At each of the time intervals total hemocyte counts and differential counts were made. Statistical comparisons of the total cell counts permit the conclusions that (1) Cu 2+ or Cu 2+ + EDTA does not appear to stimulate hemopoiesis, (2) Cd 2+ appears to stimulate hemopoiesis, (3) the stimulation of hemopoiesis by Cd 2+ does not appear to be reduced by chelation with EDTA, and (4) Cd 2+ + EDTA or EDTA alone appears to have a stimulatory effect on hemocyte production. Statistical comparisons of the differential counts permit the following conclusions: (1) 1 ppm of Cu 2+ causes a reduction in the percentage of hyalinocytes, and (2) 1 ppm of Cd 2+ causes an elevation in the percentage of hyalinocytes after 1 and 2 weeks of exposure.
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