In Vivo Dose and Duration Dependent Effects of a Dioxin (2,3,7,8 TCDD) on Few Lysosomal Enzymes in Mice Brain

Abstract

TCDD, a toxic dioxin, has a high rate of biomagnification by accumulating in adipose tissue of living organism. The present study reports that the in vivo exposure of even environmentally available low concentration of TCDD affects the lysosomal enzymes in mice brain cells. The study tested the hypothesis that low concentration of TCDD provokes dose and duration dependent effects to lysosomal enzymes in mice brain cells. Selected groups of animals were administered two very low doses of TCDD (0.004 mg/kgbw/d, 0.04 mg/kg bw/d) for 2,4 and 6 days of exposure durations. The results indicated TCDD caused significant exposure duration dependent effects to lysosomal enzymes in mice brain cells. Though it is not very clear at this point, but the results suggested that the observed alterations in the lysosomal enzymatic activity might have affected few metabolic pathways of the cell and disturbed cellular homeostasis by producing intracellular ions and ROS. These changes directly or indirectly affected the cellular metabolic pathways in different degrees depending on the dose and exposure durations of the dioxin and might have evoked apoptotic changes into the cells.