In vivo detection of L-tryptophan in cucumbers using poly (9-Aminoacridine) film modified pencil graphite electrode

Abstract

• A miniature sensor for detecting L-Trp is constructed using PAA modified PGE. • Detecting L-Trp in vivo is realized in cucumber fruit. • L-Trp levels in cucumbers in different categories are monitored. The rapid quantitative detection of L-tryptophan (L-Trp) is essential for promoting agricultural development. A miniature electrochemical sensor was constructed with the pencil graphite electrode(PGE) modified with poly (9-aminoacridine) film(PAA) for the detection of L-Trp in cucumber. The electropolymerization of PAA could greatly increase the sensitivity and selectivity, resulting in the constructed sensor with a linear range of 5-200 μM and the limit of detection as 0.035 μM. The miniature electrochemical sensor is a reliable electrochemical platform for the detection of L-Trp in cucumber fruit both in vivo and in vitro.