Abstract
In vivo corneal confocal microscopy and its operability in scientific as well as in clinical applications is often impaired by the lack of information on imaging plane position and orientation inside the cornea during patient's examination. To overcome this hurdle, we have developed a novel corneal imaging system based on a commercial scanning device and a modified Rostock Cornea Module. The presented preliminary system produces en face images by confocal laser scanning microscopy and sagittal cross-section images by optical coherence tomography simultaneously. This enables imaging guidance during examinations, improved features for diagnostics along with thickness measurements of the cornea as well as corneal substructures from oblique sections.
Highlights
Laser-based imaging modalities like scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) became indispensable for noninvasive in vivo diagnostics of the eye, especially the retina
Due to the tight focus of the RCM 2.0 objective lens, the most intense backscattered OCT signal originates from the OCT focus, which is essentially the place of the confocal microscopy (CCM) image plane. This is the reason, why the CCM image plane is visible in the OCT image and why its movement in the OCT image can be traced while changing the focus or in through focusing experiments during image stack acquisition (Fig. 3(A))
A locally dependent intensity scaling may help to increase the visibility of the surfaces in OCT images
Summary
Laser-based imaging modalities like scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) became indispensable for noninvasive in vivo diagnostics of the eye, especially the retina. Corneal confocal microscopy (CCM) and OCT became valuable tools for imaging the anterior segment of the living eye. To overcome some of these limitations, especially the latter one, we applied the multimodal retinal imaging approach to corneal imaging. In order to achieve this, we adapted a dedicated lens module to a commercially available multimodal imaging platform for the posterior segment of the eye combining SLO and OCT. The lens module shifts the en face imaging plane from the retina to the cornea in the same way as it was previously described for single modal imaging systems [4,5]
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