Abstract
After administration of holaphyllamine-4- 14C to the leaves of a Holarrhena floribunda plant, radioactive pregnenolone was isolated and shown to be radiochemically pure by chromatography, by crystallization of its acetate to constant specific activity, and by subsequent hydrolysis to free pregnenolone of the same specific activity. This experiment indicates that pregnenolone may be a natural constituent of this plant and that the previously observed conversion of pregnenolone to holaphyllamine is reversible.
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