Abstract
The present study deals with the preliminary evaluation of in vivo ciprofloxacin release from HAp-ciprofloxacin bone implants in rabbit tibia. The HAp-ciprofloxacin implants (2 × 2.5 mm) were prepared using various HAp-ciprofloxacin composites synthesized by precipitation technique and 1.5% w/v guar gum as a binder. 5 implants were implanted in artificial cortical bone window at the right proximal tibia of each rabbit. After 3- and 6-day intervals, the rabbits were euthanized. Bone marrow and serum concentrations of ciprofloxacin were determined from the harvested tibia using HPLC method. The results displayed the availability of elevated local antibiotic concentration at the implanted site with the limitation of systemic antibiotic exposure, which can be useful to minimize the risk of systemic toxicity-related side effects. This study is the preliminary investigation of the suitability of in vivo ciprofloxacin release from HAp-ciprofloxacin bone implants in rabbit tibia, after implantation, and these implants can be useful for the treatment of bacterial bone infections like osteomyelitis.
Highlights
Prolonged administration of systemic antibiotics is usually required to treat bacterial bone infections
The conventional prolonged systemic antibiotic therapy is a insufficient method for achieving high local antibiotic concentration at the diseased bone site as bones are poorly perfused organ [1]
HAp-ciprofloxacin composites were synthesized by precipitation technique using these following parameters: 2 grams of ciprofloxacin added in the synthesis process, 600 rpm of stirring speed, and 0.5 mL/minute of orthophosphoric acid addition rate in the synthesis process
Summary
Prolonged administration of systemic antibiotics is usually required to treat bacterial bone infections. Local sustained antibiotic release implantable delivery may offer considerable advantages over the traditional therapy by producing effective local antibiotic concentration at diseased site with limitation of systemic antibiotic exposure maintaining low systemic side effects [2]. Because of its biocompatibility and bone-bonding property, HAp has been used as a safe matrix in the bone drug delivery [4, 5] It offers high binding affinity for a variety of pharmacological substances such as antibiotics, hormones, and steroids [6,7,8]. A few ciprofloxacin-loaded HAp-based implantable systems developed using precipitation technique are reported [11–14]. We have developed HAp-based bone implants using synthesized HAp-ciprofloxacin composites by precipitation technique and 2% w/v aqueous solution of guar gum as binder. The in vivo ciprofloxacin release from HAp-ciprofloxacin bone implants implanted in rabbit tibia was evaluated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
