Abstract
Background: Understanding the microscopic dynamics of the beating heart has been challenging due to the technical nature of imaging with micrometer resolution while the heart moves. The development of multiphoton microscopy has made in vivo, cell-resolved measurements of calcium dynamics and vascular function possible in motionless organs such as the brain. In heart, however, studies of in vivo interactions between cells and the native microenvironment are behind other organ systems. Our goal was to develop methods for intravital imaging of cardiac structural and calcium dynamics with microscopic resolution.Methods: Ventilated mice expressing GCaMP6f, a genetically encoded calcium indicator, received a thoracotomy to provide optical access to the heart. Vasculature was labeled with an injection of dextran-labeled dye. The heart was partially stabilized by a titanium probe with a glass window. Images were acquired at 30 frames per second with spontaneous heartbeat and continuously running, ventilated breathing. The data were reconstructed into three-dimensional volumes showing tissue structure, vasculature, and GCaMP6f signal in cardiomyocytes as a function of both the cardiac and respiratory cycle.Results: We demonstrated the capability to simultaneously measure calcium transients, vessel size, and tissue displacement in three dimensions with micrometer resolution. Reconstruction at various combinations of cardiac and respiratory phase enabled measurement of regional and single-cell cardiomyocyte calcium transients (GCaMP6f fluorescence). GCaMP6f fluorescence transients in individual, aberrantly firing cardiomyocytes were also quantified. Comparisons of calcium dynamics (rise-time and tau) at varying positions within the ventricle wall showed no significant depth dependence.Conclusion: This method enables studies of coupling between contraction and excitation during physiological blood perfusion and breathing at high spatiotemporal resolution. These capabilities could lead to a new understanding of normal and disease function of cardiac cells.
Highlights
Cardiovascular disease remains a substantial burden to populations worldwide (GBD 2013 Mortality and Causes of Death Collaborators, 2015; Mozaffarian et al, 2015)
Expression of GCaMP6f was induced by intraperitoneal injection of tamoxifen in corn oil (Sigma #T5648) for five consecutive days (75 mg/kg body weight), resulting in widespread tissue expression with strong expression in cardiomyocytes
Multiphoton microscopy of GCaMP6f-expressing mice provides a novel method to study in vivo excitation-contraction coupling
Summary
Cardiovascular disease remains a substantial burden to populations worldwide (GBD 2013 Mortality and Causes of Death Collaborators, 2015; Mozaffarian et al, 2015). Better understanding of the interactions between cell contraction, blood flow, and electrical conduction in disease is needed. The elimination of motion and blood flow results in highly artificial conditions and precludes studies of the correlation between local vascular and tissue function possible in other tissues such as the brain (Shih et al, 2012; Cianchetti et al, 2013). Understanding the microscopic dynamics of the beating heart has been challenging due to the technical nature of imaging with micrometer resolution while the heart moves. The development of multiphoton microscopy has made in vivo, cell-resolved measurements of calcium dynamics and vascular function possible in motionless organs such as the brain. Our goal was to develop methods for intravital imaging of cardiac structural and calcium dynamics with microscopic resolution
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
