Abstract
The effective medical applications of cold atmospheric pressure plasma jet (APPJ) have been reported by many researchers, including sterilization of liquid and solid surfaces, treatment of chronic wounds, treatment of cancer tumors, and blood clots. Therefore, in this study, we exposed Aporrectodea trapezoides and Eisenia fetida earthworms to APPJ discharge (0-30s) to evaluate the impacts on regeneration of missed segments, apoptosis, lipid peroxidation (LPo), catalase activity (CAT), total antioxidant capacity (TAC), total proteins, and protein profile. Results showed APPJ induced significant effects on regeneration ability of earthworms after 20 and 30s of exposure (p < 0.05). Atmospheric pressure plasma jet did not have significant effects on MDA content and TUNEL-positive cells, but this effect was significant for TAC and CAT in both species (p < 0.05). In conclusion, the present study revealed for the first time that regeneration of missed segments in earthworms can be stimulated by plasma treatment.
Highlights
Plasma, as the fourth state of matter, is an ionized gas capable of producing reactive species of oxygen, nitrogen, free radicals, electrons, ions, and neutral particles (Lee et al 2009; Lu et al 2016)
This study investigated the effects of atmospheric pressure plasma jet (APPJ) discharge on the two species of Earthworms (Aporrectodea trapezoides and Eisenia fetida)
It can be concluded that APPJ exposure up to 40 seconds didn’t inhibit effects on the total antioxidant capacity (TAC) levels of the earthworms and the defense system was able to fight oxidative stress and by increasing the expression of some proteins APPJ lead to better regeneration
Summary
As the fourth state of matter, is an ionized gas capable of producing reactive species of oxygen, nitrogen, free radicals, electrons, ions, and neutral particles (Lee et al 2009; Lu et al 2016). ROS include superoxide anion (O2−), hydroxyl radical (OH), hydrogen peroxide (H2O2), nitric oxide (NO), peroxynitrite (ONOO−), and so on (Kalghatgi et al 2011)
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