Abstract
Dialium angolense is used in traditional medicine in Bagira-Bukavu in the management of malaria. In this study, in vivo antiplasmodial and in vitro antioxidant activities, phytochemical screening of secondary metabolic and in vivo toxicological studies where carried out on aqueous and methanolic extracts of its leaves. The plant was selected following an ethnobotanical survey conducted in DR Congo and focusing on antimalarial plants. Extracts’ phytochemical secondary metabolites were determined using standard procedures and the antiplasmodial activity was evaluated using 4-day suppressive test, while antioxidant activity was evaluated by DPPH assay. In acute toxicity, eighteen animal (6/group) were given orally singular 2000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assay, 150 or 300 mg/kg BW/Day were given orally, and animals (6/group) were observed for 28 days. The total phenolic (0.89 - 0.98 mg GAEg-1), total flavonoid (0.42 - 0.44 mg QEg-1) and total tannin contents (0.080 – 0.098 mg GAEg-1) were in the same rate in the two extracts as well as the antioxidant activity with IC50 value 6.1 and 6.8 μg/mL. At the highest oral dose, 300 mg/kg body weight, all extracts produced 70.4–70.8% chemo-suppression against P. berghei ANKA and 28 survival days. No deaths were recorded during the acute toxicity assay suggesting the LD50 > 2000 mg/kg and no abnormal behavior or variation in toxicity biomarkers were observed during the subacute toxicity assessment. D. angolense leaves extracts showed a great antiplasmodial and a very good antioxidant activity. It can be used to prepare antimalarial recipe or isolate antimalarial compounds in the future.
Highlights
Malaria is one of the most prevalent and serious protozoan tropical diseases which causes millions of clinical cases worldwide each year, and approximately 1 million of death annually [1]
Phytochemical screening of Dialium angolense leaves extracts The phytochemical screening of D. angolense leaves extracts revealed the presence of quinones, flavonoids, polyphenols, terpenoids, but the absence of alkaloids, coumarins, steroids, and saponins (Table 1)
The different total phenolics content (TPC) values vary between 0.89-0.982 mg GAEg-1 and are two times higher than the total flavonoids content (TFC values: 0.44 - 0.42 mg QEg-1)
Summary
Malaria is one of the most prevalent and serious protozoan tropical diseases which causes millions of clinical cases worldwide each year, and approximately 1 million of death annually [1]. Many Congolese people do not have access to modern health care and use medical plants but, many plants used in traditional medicine have not been studied [5] Another element of the antimalarial control strategy would consist in validating the use of antimalarial plants with the hope to discover new compounds or produce improved traditional drugs. 2.5 cm × 1.5 cm, densely dark brown hairs, with greenish-white pulp, sepals persistent at base, indehiscent, 1 (–2) -seed. The fruits, beyond being edible, are used in the treatment of pneumonia [4,13] This plant has already been investigated for in vitro antiplasmodial activity, study which revealed a great activity [4]. No information is reported on its phytochemical composition nor on the in vivo antimalarial activity, and toxicity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: World Journal of Biology Pharmacy and Health Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
