Abstract
Online electrochemical systems (OECSs) possess a distinct continuously monitoring ability toward electro-active species and have been intensively used for single/multiple neurochemical measurements. However, detecting electro-inactive species or some substances being oxidized/reduced at close potentials is difficult in OECS, and it is highly demanded to broaden such useful online methodology to more new principles, which, however, is still lacking. The system reported in this Letter demonstrates a solution to the aforementioned problem. We report an online optical detection platform (OODP) based on visible light absorption for the continuous monitoring of neurochemicals in rat brains with ascorbic acid (AA) as an example. The OODP is constructed with a homemade online capillary-based microfluidic line as the optical detector built under a brightfield microscope. Cobalt oxyhydroxide (CoOOH) nanoflakes directly oxidizing 3,3′,5,5′-tetramethylbenzidine (TMB) into a blue-colored product (oxTMB) which can be f...
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