In vivo and in vitro study of immersion clearing dynamics of the skin

Abstract

Experimental results on in vivo and in vitro immersion clearing dynamics of the skin under action of the osmotic agent are presented. The significant decrease of the human skin reflectance in vivo under action of glucose solution is demonstrated. In vitro experiments with dyed immersion agents have shown that the immersion mostly penetrates into skin tissue through the dermal layer. Diffusion of the immersion agent through the stratum corneum barrier was insignificant. It was shown that hypodermic injection of the immersion agent is an effective way for the agent administration. The time-dependent contrast of the blood perfused areas in the cleared skin was estimated. The diffusion coefficient of 40%-glucose solution in skin in vivo was estimated as D = (2.56 ± 0.13)·10-6 cm2/sec.