Abstract
Background: Ultrasound-targeted microbubble destruction has been utilized to deliver a drug/gene into cells in vitro and in vivo. Objective: To access the effects of ultrasound or/and microbubbles on recombinant adeno-associated virus (rAAV)-mediated transgene expression in rat retinal pigmented epithelium-J (RPE-J) cells in vitro and Wistar rat retina in vivo and to compare the difference between them. Methods: Different doses of serotype-2 rAAV (rAAV2) encoding an enhanced green fluorescent protein gene and microbubbles were administered to RPE-J cells in vitro and Wistar rat retina in vivo under different ultrasound conditions. Transfection efficiency was assessed by fluorescence microscopy, fluorescence stereoscope and flow cytometry analysis. Cell viability and tissue damage were assessed by trypan blue staining and hematoxylineosin staining. Results: rAAV2-mediated transgene expression in vivo was significantly enhanced by ultrasound and microbubbles, while rAAV2-mediated gene transfection in vitro was significantly enhanced by ultrasound or microbubbles alone. Conclusion: Ultrasound or/and microbubbles-mediated rAAV delivery strategies must be chosen for retinal gene therapy based on their effects in vitro and in vivo.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
