In-Vivo And Histological Toxicity Profiling Of A Natural Phenol: Phloretin

Abstract

Phloretin is a flavonoid, which is having strong antioxidant potentials and used in the treatment of numerous disorders. The aim of this study to evaluate the possible toxicological effect of Phloretin in Swiss albino mice. Methods: Single dose of Phloretin was administered stepwise in two groups by oral route and general behaviour, adverse effects and mortality were determined up to 14 days and compared to normal group. In sub-acute study, Phloretin was administered orally for 28 days to the four experimental groups and their body weight was measured each alternate day from the first day of dosing. The final body weight was recorded on 29th day and animals were euthanized by using thiopentone sodium, blood was collected and later haematological, lipid profile, biochemical parameter was evaluated and compared to normal group. Result: In acute toxicity study, no abnormal general behaviour, adverse effects were reported. The body weight, haematological, lipid profile and biochemical parameter also not recorded with any significant changes in sub-acute toxicity study. No significant mortality was reported in both the study. Histopathological examination revealed no alterations in clinical signs or organ weight at any dose. Conclusion: The result concludes that the oral administration of Phloretin did not produce any significant toxic effect in swiss albino mice. Hence, the test drug Phloretin is safe for therapeutic uses.