Abstract
A simple fluorescence microscopy was established using carboxyfluorescein diacetate (CFD) for labeling mouse lymphocytes. By syngeneic transfer and subsequent examination of the adherence of lymphocytes to high endothelial venules (HEVs) of Peyer’s patches (PPs) and peripheral lymph nodes (PLNs), the CFD-labeled lymphocytes were found to be superior to those labeled by tetramethylrhodamine isothiocyanate (TRITC) or by fluorescein isothiocyanate (FITC) in fluorescence intensity and longevity. PLN lymphocytes and T cells are superior to PP lymphocytes and T cells in adherence to HEVs of both PPs and PLNs. Both B cells of PPs and PLNs tend to adhere to HEVs of PPs better than to HEVs of PLNs.
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