Abstract
The present investigation was undertaken for developing a high frequency regeneration system, as an essential pre-requisite for the genetic transformation in brinjal. Regeneration of multiple shoots via callus induction and organogenesis was achieved using cotyledons, hypocotyls and roots as explants at various concentrations and combinations of growth regulators. Hundred percent callus induction was observed in cotyledon and hypocotyl explants when cultured on medium supplemented with BAP or Zeatin. Earliest callus induction was observed in cotyledon explants (8.03 days). The MSB5 media supplemented with 2 mg/l zeatin + 0.1 mg/l IBA found to be the best medium with regards to number of days requires for callus induction and shoot induction, number of shoot buds formed per explant and regeneration frequency. Among explants used cotyledons found to be give best response to shoot induction and number of shoot bud formation (87.50% and 24) and followed by hypocotyls (83.33% and 22) and root explant (69.17% and 12). In vitro rooting (53.33%) was obtained within 13 days on MSB5 media supplemented with 1mg/l IBA. The in vitro rooted plantlets were successfully established in polycabonated polyhouse with 92% survival rate.
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