In vitromodelling of salinity stress for the selection of stress-tolerant birch lines

T M Tabatskaya,O S Mashkina,O M Korchagin,V Breskich,Y Dreizis,A Zheltenkov

doi:10.1051/e3sconf/202022404013

T M Tabatskaya, O S Mashkina + Show 4 more

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https://doi.org/10.1051/e3sconf/202022404013

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Abstract

This paper examines the salinity stress effectin vitroculture of microshoots of 10 birch clones:Betula pendulaL.,B. pendulaRoth var.carelica(Mercklin) Hämet-Ahti,B. pendulaf. ’dalecarlica’(L.f.) Schneid.,B. pubescensEhrh. We used economically valuable and genetically diverse (various species, varieties, hybrids, polyploids) birch material fromin vitroclone collection. We tested 3in vitroselection methods on 1/2 MS hormone-free nutrient medium: mild – with a prolonged exposure to 0.2% NaCl; gradual – with a step-by-step increase in osmotic concentration (from 0.2% to 1%); severe – with a sublethal (1%) NaCl concentration. We established that the gradual method of NaCl exposure according to the following pattern: 0.2% → 0% → 0.5% → 0% → 0.75% → 0% → 1.0% resulted in the best selective effect for birch, as it provides a clear differentiation of clones in terms of their salinity tolerance. We identified that the genotype had a significant impact on the preservation of the explants and their ability to regenerate under salinity stress. The study showed that tetraploid clones of downy birch and a triploid clone of Karelian birch of a pronounced mixoploid nature had the greatest salinity tolerance.

Highlights

IntroductionAbiotic stress (drought, soil salination, soil pollution by heavy metals etc.) has a negative impact on growth, development and productivity of plants (including forest trees) [1,2]
Abiotic stress has a negative impact on growth, development and productivity of plants [1,2]
We established that the gradual method of NaCl exposure according to the following pattern: 0.2% → 0% → 0.5% → 0% → 0.75% → 0% → 1.0% resulted in the best selective effect for birch, as it provides a clear differentiation of clones in terms of their salinity tolerance

Summary

Introduction

Abiotic stress (drought, soil salination, soil pollution by heavy metals etc.) has a negative impact on growth, development and productivity of plants (including forest trees) [1,2]. There are reports of drought deaths in Eucalyptus globulus (Labill.) plantations in Australia [4]. In this regard, plants with improved stress tolerance are becoming more and more popular. Genetic diversity in plant species is a prerequisite for the perspective of successful breeding for stress resistance. Genotypic differences in salinity tolerance have been found in poplar [6,7] and other plants [8,9]

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