Abstract
One highly desirable objective of preventive medicine is to find predictive markers that could identify neonates who are at risk of developing atopic diseases. However, no one laboratory test is sufficiently sensitive and specific to be used for this purpose. The objective of this study was to evaluate the use of an in vitro IgE synthesis by cord blood lymphocytes of neonates with or without a family history of atopy. Cord blood mononuclear cells (CBMC) of 11 neonates with a family history of atopy and 9 neonates without a family history of atopy were incubated for 48 hr with human recombinant IL4 (hrIL4) in Petri plates. Cord blood lymphocytes (nonadherent cells) were then washed and incubated for an additional 9 days with various stimuli. hrIL4, hrIL4 + human recombinant interferon-gamma (hrIFNg), hrIL4 + anti-IL4 antibody (ab), hrIL-4 + antiCD40 ab, hrIL4 + anti-CD40 ab + betalactoglobulin (BLG) hrIL4 + anti-IFNg ab. Unstimulated cultures were included as negative controls. IgE concentrations in sera w...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
