Abstract
A successful technique was developed for micrograftirig resting-bud and growing-tip scions of three Picea spp. (P. abies (L.) Karst, P. pungens f. glauca (Regel) Beissn. (and its clone ‘Koster’) and P. sitchensis Bong) on P. pungens f. glauca and P. sitchensis rootstocks. In vitro rootstock production under both sterile and non-sterile conditions and micrografting using two types of graft (apical and side) are described. Both types of graft were equally successful although apical grafts were preferred since the additional operation of removing at four to five weeks the epicotyl above the graft following union was unnecessary. Culture medium strength was found to affect micrografting success rates: 1/3- strength B5 medium with 2% (w/v) sucrose giving the best results. Antioxidant additives applied to the micrograft unions were found to reduce significantly union success rates whilst the physiological age of scions used had no effect on graft union successes. A modified micrografting method was developed fr...
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