In vitroeffect of N-desulfated heparin on the expression of basic fibroblast growth factor in gastric carcinoma cell line SGC-7901

Abstract

AIM: To investigate the effect of N-desulfated heparin on the expression of basic fibroblast growth factor (bFGF) in human gastric carcinoma SGC-7901 cells in vitro. METHODS: Human gastric carcinoma cell line SGC-7901 was cultured in vitro, and then treated with N-desulfated heparin at different concentrations (0.1, 1.0 g/L). Meanwhile, SGC-7901 cells cultured in single RPMI 1640 medium were used as controls. Each group contained 3 paralleled samples. Enzyme-linked immunosorbent assay (ELISA) and real time polymerase chain reaction (PCR) were used to detect the expression of bFGF in SGC-7901 cells at 12 h and 24 h, respectively. RESULTS: After treatment with 0.1 or 1.0 g/L N-desulfated heparin for 12 or 24 h, bFGF protein expression was decreased significantly (t=7.502, P=0.002; t=55.416, P=0.000; t=52.221, P=0.000; t=48.080, P=0.000). The expression of bFGF (CT value) in each N-desulfated heparin group was higher than that in the control group at the same time. The inhibition of N-desulfated heparin on bFGF protein and mRNA expression in SGC-7901 cells was dose-and time-dependent. CONCLUSION: N-desulfated heparin can inhibit the expression of bFGF in gastric cancer cell line SGC-7901 in a dose- and time-dependent manner in vitro.