Abstract
One of the major skin lipids, triolein, was irradiated by 300 nm ultraviolet (UV) light under conditions approximating those at the skin surface exposed to sunlight. Irradiated samples were analyzed for acrolein, formaldehyde, and acetaldehyde by gas chromatography. The acrolein formed was derivatized to more stable l-methyl-2-pyrazoline with N-methylhydrazine and was analyzed by a nitrogen-phosphorus-specific detector. Formaldehyde and acetaldehyde formed were reacted with cysteamine to give thiazolidine and 2-methylthia-zolidine, respectively, and were analyzed by a flame photometric sulfur-specific detector. The maximum amount of acrolein (1.05 nmol/mg triolein) formed after 6 hr of irradiation. The maximum quantities of formaldehyde (6 nmol/mg triolein) and acetaldehyde (2.71 nmol/mg triolein) formed after 12 hr of irradiation. Both formaldehyde and acrolein have been known to cause skin irritation in concentrations of 1 ppm.
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