Abstract
A standard protocol for an efficient in vitro micropropagation of Pulicaria crispa, a medicinally important plant, was designed. The in vitro plantlet production was investigated on Murashige and Skoog (MS) medium with various combinations of auxins and cytokinins. From nodal explants, maximum number of shoots (3 shoots) and beneficial shoot length (3.9 cm) were achieved on MS medium containing 0.5 mg L–1 BAP. MS media with the combination of BAP (0.5–1 mg L−1) and KN (0.5–1 mg L−1) showed maximum response of shoot number and mean shoot length. Rooting of well developed shoot was excellent on half strength MS medium after dipping in 1 mg L−1 IBA for 30 min. The well rooted plantlets were then hardened with high survival rate. This is the first report dealing with in vitro regeneration for P. crispa.
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