Abstract
The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3 × 104 cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hour incubation with 5% CO2 at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2. Cells were irradiated every 24 h totalizing 3 applications. Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay). Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%. Irradiation of the fibroblasts with 0.5 and 3 J/cm2 resulted in significant increase in cell metabolism compared with the nonrradiated group (P < 0.05). Both energy doses promoted significant increase in the cell number as well as in cell migration (P < 0.05). These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.
Highlights
Tissue healing involves an intense activity of diverse cell types, such as epithelial and endothelial cells, as well as fibroblasts which play a key role in this process [1]
Irradiation of the fibroblast cultures with doses of 0.5 J/cm2 and 3 J/cm2 resulted in 11% and 17% increases in cell metabolism, respectively, differing significantly from the control group (P < 0.05)
Establishing the ideal irradiation parameters and techniques is mandatory for the development of sequential studies that can determine the potential biostimulatory effect of level laser therapy (LLLT) on oral mucosa cells, such as keratinocytes and fibroblasts, which are directly involved in the local healing process
Summary
Tissue healing involves an intense activity of diverse cell types, such as epithelial and endothelial cells, as well as fibroblasts which play a key role in this process [1]. Fibroblasts secrete multiple growth factors during wound reepitelialization and participate actively in the formation of granulation tissue and the synthesis of a complex extracellular matrix after reepitelialization [1]. All these processes directly involve the proliferation and migration capacity to these cells [1]. The aim of this study was to determine the most adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast cultures in an in vitro wound healing model
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