In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response

Abstract

Expression of the hypersensitive response (HSR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum L. cv. Xanthi-nc (genotype NN) is controlled by the single dominant “N” gene and is temperature sensitive. TMV-infected Xanthi-nc tobacco plants grown at the HSR-restrictive temperature of 31° for 3 days postinoculation show necrosis approximately 8 hr after the temperature shift to the HSR-permissive temperature of 25°. Both polyribosomal and total cytoplasmic poly(A)-containing RNAs were isolated at various times after the temperature shift from leaves of TMV-infected and mock-infected Xanthi-nc tobacco plants and from TMV-infected Turkish Samsun tobacco plants (genotype nn; systemic for TMV infection). The RNAs were translated in vitro and the products were analyzed by polyacrylamide gel electrophoresis. A minimum of four polypeptide translation products specific to both the polyribosomal and total cytoplasmic poly(A)-containing RNAs derived from hypersensitively responding Xanthi-nc were observed.