In vitro translation of the genome of a small RNA virus from Trichoplusia ni

Abstract

The genomic RNA of a member of the “ Nudaurelia β virus” group functioned as a mRNA in vitro. The translation products included a protein, which comigrated with the single virus capsid protein, and a stable 100 × 10 3 MW protein, which was synthesized by cleavage of a precursor protein. No precursor proteins were involved in synthesis of the putative capsid protein. Attempts to inhibit proteolytic cleavage did not result in the appearance of a product corresponding to the entire coding capacity of the genome.