Abstract

In vitro transformation of insect plasmatocyte have been studied in physiological saline-versene, tissue culture, and the haemolymph. Transformation of a plasmatocyte into a granular cell can occur either by fragmentation of portions of the cytoplasm or by a gradual rounding-off of the fusiform plasmatocyte. The spherule cell is derived either from a plasmatocyte directly or from a granular cell. Cystocytes are formed from round plasmatocytes or from granular cells. Although gradual transformation of a plasmatocyte and a spherule cell into an adipohaemocyte was not observed, a granular cell was found changing into an adipohaemocyte. Oenocytoids are formed from fusiform plasmatocytes by cytoplasmic fragmentation. Podocytes and vermiform cells are derived from fusiform plasmatocytes by gradual transformation. All these transformations seem to be governed by specific physiological conditions. Oenocytoid- and lamellocyte-like cells are present in the haemolymph of Periplaneta americana (L.). The polymorphic plasmatocyte is the basic type of haemocyte in insects.

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